- Ukwelashwa ngama-nanoparticles e-bioactive kusebenza kusivimbeli segazi nobuchopho hhayi ngokuqondile kuma-neurons.
- Kumamodeli egundane, ukuncipha kwe-50-60% ku-amyloid kwafinyelelwa ngesikhathi somjovo kanye nokuthuthukiswa kwengqondo ngemva kwemithamo emithathu.
- Izinhlayiya zilingisa imigqa ye-LRP1, iphinde isebenze indlela yemvelo yokususa, futhi ikhuthaze ukuqedwa kwe-Aβ egazini.
- Le ndlela, eshicilelwe ku-Signal Transduction and Targeted Therapy, iyathembisa kodwa isadinga ukuhlolwa kwabantu.
Un iqembu lamazwe ngamazwe, nobuholi be-Institute of Bioengineering of Catalonia (IBEC) kanye neSibhedlela saseWest China saseSichuan University, wethule isu le-nanotechnology ukuthi ibuyisela emuva izimpawu ze-Alzheimer's kumagundane ngokulungisa umgoqo wegazi ebuchosheni (BBB). Uma sikhuluma ngobubanzi, imayelana sebenzisa ama-nanoparticles asebenza njengezidakamizwa ngokwawo ukuze buyisela umsebenzi we-cerebral vascular.
Lokhu kushintsha kokugxila kunengqondo uma sikhumbula lokho ubuchopho budla cishe Amaphesenti angu-20 wamandla kubantu abadala ngisho 60% ezinganeni, esekelwa inethiwekhi eminyene yama-capillaries lapho i-neuron ngayinye ithola ukwesekwa. Uma i-BBB ishintshwa, uhlelo lokulahla imfucuza luyahlupheka futhi luthanda ukuqoqwa kwe-beta amyloid (Aβ), uphawu oluphawulekayo lwe-pathology.Kulinganiselwa ukuthi ubuchopho bomuntu buqukethe cishe ama-capillary ayizigidi eziyinkulungwane, yingakho ukubaluleka kwempilo yemithambo.
Lelisu le-nanotechnology liphakamisa ukuthini?
Ngokungafani ne-nanomedicine yakudala, esebenzisa ama-nanoparticles njengezimoto nje, le ndlela iyasebenza izidakamizwa ze-supramolecular okuyizinto eziphilayo futhi ezingadingi ukuthutha esinye isimiso. Okuhlosiwe akuyona i-neuron, kodwa i-BBB njengenhloso yokwelapha.
Ngaphansi kwezimo ezijwayelekile, I-LRP1 receptor ibona i-Aβ futhi iyidlulisele ngaphesheya komgoqo iye egaziniIsistimu, nokho, ithambile: Uma ukubopha kudlulele noma kunganele, ezokuthutha azilingani futhi i-Aβ iyanqwabelana. Ama-nanoparticles aklanyelwe ukulingisa LRP1 ligands ukubuyisela lokho kulinganisela.
Ngalokhu kungenelela, umzila wokuphuma wamaprotheni ayinkinga kusuka ku- i-parenchyma egazini, ikhuthaza ukucaciswa kwe-Aβ kanye nomsebenzi wesithiyo ojwayelekile. Ngamafuphi, ivuselela i- umzila wokuhlanza wemvelo yobuchopho.
Ukuhlolwa kwemodeli yezilwane kanye nemiphumela
Ukuhlola kwenziwe kumagundane ashintshwe izakhi ukuze kukhiqizwe inani elikhulu le-Aβ futhi kuthuthukiswe ukukhubazeka kwengqondo. Imijovo emithathu yalezi zinhlayiya yayanele ukubona izinguquko ezilinganisekayo kuma-biomarker nokuziphatha..
Ngokusho kwababhali, ihora elilodwa nje emva kokuphatha Ukwehla ngo-50-60% ku-Aβ ebuchosheni sekuvele kurekhodiweUkushesha komphumela kuphakamisa ukwenziwa kusebenze kabusha ngokushesha kwendlela yokuthutha kuwo wonke umgoqo.
Ngaphandle komthelela osheshayo, imiphumela ehlala njalo iyachazwa. Kokunye ukuhlola, igundane elinezinyanga ezingu-12 liphinde lahlaziywa ezinyangeni ezingu-18 futhi laboniswa. ukusebenza okufana nokwesilwane esinempilo, okubonisa ukululama okuqhubekayo kokusebenza ngemva kokwelashwa.
Iqembu lihumusha ukuthi kukhona a umphumela we-chain: ngokubuyisela ukusebenza kwe-vascular, Ukucaciswa kwe-Aβ namanye ama-molecule ayingozi kuyaqala futhi, futhi isistimu ithola ibhalansi yayo.. Emazwini obuholi besayensi, izinhlayiya zisebenza njengesidakamizwa esinjalo ivuselela indlela yokususa kumazinga ajwayelekile.
Ongoti bangaphandle bachaza ukutholakala njengokuthembisayo, nakuba beveza ukuthi imiphumela isitholakele kumamodeli we-murine futhi lokho kuhumusha ezigulini kudinga ukuqapha. Umphakathi ugcizelela isidingo sokuqinisekisa ukuphepha nokusebenza ngempumelelo kubantu ngezifundo eziqinile.
Ubunjiniyela be-molecular ngemuva kwe-nanoparticles
Lawa ma-nanoparticles akhulelwa ngendlela ye ubunjiniyela bamangqamuzana aphansi phezulu, ukuhlanganisa usayizi olawulwayo no-a inombolo echaziwe yama-ligands ebusweni bayo ukuxhumana nama-receptors ngendlela ethile.
Ngokushintsha i- ithrafikhi yokwamukela ku-membrane, Izinhlayiya zishuna kahle inqubo yokudluliselwa kwe-Aβ kuyo yonke i-BBBLeli zinga lokunemba livula izindlela zokuthi lawula imisebenzi yama-receptor okwakunzima kuze kube manje ukukusebenzisa ngendlela yokwelapha.
Ngakho-ke, akukhuthazwa kuphela ukuqedwa okusebenzayo kwe-Aβ, kodwa Isiza ukulinganisa amandla e-vascular asekela ukusebenza kobuchopho obunempilo.. Lona umehluko oyinhloko ezindleleni ezilinganiselwe ukuletha imithi.
Ubani obambe iqhaza futhi yini elandelayo?
I-consortium ihlanganisa ndawonye I-IBEC, Isibhedlela sase-West China kanye neSibhedlela sase-Xiamen West China sase-Sichuan University, the Ikolishi LaseNyuvesi LaseLondon, i Universidad de Barcelona, ICREA, kanye ne-Chinese Academy of Medical Sciences, phakathi kokunye. Okutholakele kushicilelwe kujenali Ukuguqulwa Kwesiginali kanye Nokwelashwa Okuhlosiwe.
Ngokubheka ukuhumusha, uhambo olunengqondo luyadlula ukuqinisekiswa okuzimele, Izifundo ze-Toxicological, ukuhlaziywa komthamo kanye, uma kufanele, ukuhlolwa kwabantu kwesigaba I/IIUkuphepha nokukhiqiza kabusha kuzoba ukhiye wokuqhubekela phambili.
Ngaphandle kwe-Alzheimer's, lo msebenzi ugxile ku- impilo ye-cerebrovascular njengengxenye eyinhloko yokuwohloka komqondo, ukuvula inkambu yokwelapha ehambisana nezindlela zakudala ze-neuron-centered.
Isethi yedatha iphakamisa ukuthi ukungenelela kusithiyo sobuchopho begazi nge ama-nanoparticles e-bioactive inganciphisa ngokushesha umthwalo we-amyloid, ibuyisele ukusebenza kwe-vascular, futhi ithuthukise imiphumela yokuqonda kumagundane; indlela ethembisayo okufanele, ngokuqapha okufanele, iqinisekiswe kuyo izifundo zomtholampilo yakhelwe kahle.
Ngingumshisekeli wezobuchwepheshe oguqule izintshisekelo zakhe "ze-geek" zaba umsebenzi. Ngichithe iminyaka engaphezu kwengu-10 yempilo yami ngisebenzisa ubuchwepheshe obusezingeni eliphezulu kanye nokukitaza ngazo zonke izinhlobo zezinhlelo ngenxa yelukuluku lokufuna ukwazi. Manje sengiqeqeshelwe ubuchwepheshe be-computer nemidlalo yama-video. Lokhu kungenxa yokuthi sekuphele iminyaka engaphezu kwengu-5 ngisebenza ngokubhalela amawebhusayithi ahlukahlukene ezobuchwepheshe nemidlalo yevidiyo, ngenza izindatshana ezifuna ukukunikeza imininingwane oyidingayo ngolimi oluqondakala yiwo wonke umuntu.
Uma unemibuzo, ulwazi lwami lusukela kuyo yonke into ehlobene nesistimu yokusebenza ye-Windows kanye ne-Android yomakhalekhukhwini. Futhi ukuzibophezela kwami kuwe, ngihlala ngizimisele ukuchitha amaminithi ambalwa futhi ngikusize uxazulule noma yimiphi imibuzo ongase ube nayo kulo mhlaba we-inthanethi.